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HomeEdexcel Past Paper PracticeBiologyEdexcel IGCSE Biology Paper 2 May 2024: Exam Guide & MCQ Quiz

Edexcel IGCSE Biology Paper 2 May 2024: Exam Guide & MCQ Quiz

Quick answer: This is the Pearson Edexcel International GCSE Biology paper 4BI1 1BR from May 2024, worth 110 marks in 2 hours. This quiz has 36 new multiple-choice questions on cells, enzymes, breathing, ecology, genetics, biotechnology and practical skills, each with a full explanation.

Last reviewed: November 2026.

What is in Edexcel IGCSE Biology Paper 2 May 2024?

PartWhat it testsMarks
Question 1Cell structure, tissues, dilution and osmosis in onion cells7
Question 2Flower structure, magnification, pollen adaptation, cuttings8
Question 3Breathing model, airways, alveoli, standard form, CO2 experiment14
Question 4Amylase investigation, starch test, mean, temperature and enzymes13
Question 5Arctic food web, trophic levels, percentage increase, plankton biomass11
Question 6Reflex arc, snail habituation, graph plotting and description13
Question 7Artery and vein structure, coronary heart disease, risk factors graph13
Question 8Lactose intolerance genetics, natural selection, yoghurt fermenter14
Question 9Genetic engineering, photosynthesis limiting factors, glasshouse system11
Question 10Designing a scarecrow colour investigation6

How can you score higher on this paper?

  • Check the mark allocation: a 2-mark ‘explain’ needs two linked points, such as a cause and its effect.
  • In calculations, convert units first (mm to µm) and show every step so you can earn method marks.
  • For ‘describe an investigation’ answers, remember CORMMS: change, other variables kept the same, repeat, measure, time, same conditions.
  • Use data from graphs and tables in your answer, with numbers and units, when asked to comment or discuss.
  • Name the exact structure or term (osmosis, denatured, restriction enzyme, ligase) because the mark scheme underlines key words.

Practice quiz: 36 multiple-choice questions

Choose one answer for each question, then press Finish to see your score and a full explanation for every answer. The questions follow the order of the paper.

These papers are designed to help students and some mistakes may exist. If you find one, please tell us so we can correct it.

0 of 36 answered
Question 1Paper ref 1(a)(i)KnowledgeA technician compares mature human red blood cells with onion epidermis cells under a microscope. Which statement about the human red blood cells is correct?
Question 2Paper ref 1(b)(i)ApplicationA student has a 10% sucrose solution and pure water. She needs 20 cm³ of a 2% sucrose solution. Which method makes it?
Question 3Paper ref 1(b)(ii)ApplicationRed onion epidermis cells are left in a strong sucrose solution for one hour. Under the microscope the coloured cytoplasm has pulled away from the cell wall, which keeps its shape. Which explanation is correct?
Question 4Paper ref 2(a)KnowledgeIn an insect-pollinated flower one part makes pollen and another part becomes the fruit after fertilisation. Which row is correct?
Question 5Paper ref 2(b)(i)ApplicationA pollen grain appears 36 mm wide in a microscope image. Its actual diameter is 240 µm. [1 mm = 1000 µm] What is the magnification, to the nearest whole number?
Question 6Paper ref 2(c)ApplicationA gardener has one plant with unusual deep red flowers. She wants many plants with exactly the same flowers, quickly, in winter when no insects are around. Why are cuttings better than growing seeds?
Question 7Paper ref 3(a)(i)-(ii)ApplicationIn a bell-jar model of breathing, balloons inside the jar are joined to the outside air by a tube, and a rubber sheet seals the bottom. The sheet is pulled down. Which row says what the sheet represents and what happens inside the jar?
Question 8Paper ref 3(a)(iii)AnalysisWhich is a valid reason why the bell-jar model does not accurately show human ventilation?
Question 9Paper ref 3(b)(ii)ApplicationA lung has 350 000 000 alveoli. Each alveolus has a surface area of 0.0060 mm². What is the total surface area in mm², in standard form?
Question 10Paper ref 3(b)(iii)ApplicationWhich statement correctly links a feature of an alveolus to how it maximises gas exchange?
Question 11Paper ref 3(b)(iv)ApplicationA student times how long it takes for limewater to turn cloudy as they breathe out through a straw, first at rest and then after two minutes of running. Which plan gives the fairest comparison of the rate of carbon dioxide release?
Question 12Paper ref 4(a)(i)-(ii)KnowledgeAmylase digests starch. Which row gives the product of this digestion and the parts of the body that make amylase?
Question 13Paper ref 4(b)(ii)AnalysisSamples of a starch and amylase mixture are tested with iodine solution every five minutes. At 20 minutes the sample turns blue-black. At 25 minutes it stays orange-brown. What does this show?
Question 14Paper ref 4(c)(i)ApplicationIn an amylase investigation at 35 °C the time for all starch to be digested was measured in three runs:
Run 1: 14 minutes
Run 2: 16 minutes
Run 3: 16 minutes
What is the mean time to two significant figures?
Question 15Paper ref 4(c)(ii)AnalysisIn an amylase investigation, the time to digest all starch fell from 48 minutes at 10 °C to 12 minutes at 40 °C. Which explanation is correct?
Question 16Paper ref 4(c)(iii)ApplicationAt 50 °C starch was still present in an amylase mixture long after the tests at lower temperatures had finished. What is the best explanation?
Question 17Paper ref 4(c)(iv)EvaluationAn amylase investigation gave digestion times of 23 minutes at 30 °C, 12 minutes at 40 °C, and no digestion at 50 °C. How could the student more accurately find the temperature at which amylase works fastest?
Question 18Paper ref 5(a)(i)KnowledgePhytoplankton are protoctists that photosynthesise. Which pair of features do they share with plant cells?
Question 19Paper ref 5(a)(ii)-(iii)ApplicationPart of an ocean food web has these feeding links (arrow means ‘is eaten by’):
phytoplankton → zooplankton
phytoplankton → plankton-eating fish
zooplankton → plankton-eating fish
zooplankton → grey whale
plankton-eating fish → grey whale
plankton-eating fish → predatory fish
plankton-eating fish → seal
predatory fish → seal
predatory fish → polar bear
seal → polar bear
Which food chain in this web has the most trophic levels?
Question 20Paper ref 5(b)(i)ApplicationThe biomass of phytoplankton in the water increased from 2.5 mg per dm³ to 17.5 mg per dm³ in one month. What is the percentage increase?
Question 21Paper ref 5(b)(ii)AnalysisIn an ocean, phytoplankton biomass peaks sharply in spring. In early summer the biomass drops steeply, while hours of light per day are still high, nitrate concentration is very low and zooplankton biomass is rising. What best explains the fall in phytoplankton?
Question 22Paper ref 6(a)ApplicationA person’s finger touches a hot object and the hand pulls away automatically. Which sequence shows the pathway of the nerve impulse in the reflex arc?
Question 23Paper ref 6(b)(ii)AnalysisA student touches a snail’s eyestalk eight times and records how long the snail stays in its shell after each touch. Which description of the trend in the chart above is correct?
Time snail stays in shell
05101512345678Stimulus numberTime (s)
Show the chart data as a table
Stimulus numberTime in shell (s)
115
215
314
415
512
68
75
83
Question 24Paper ref 6(b)(iii)EvaluationA snail withdraws into its shell for a shorter time each time its eyestalk is touched repeatedly with a cotton wool bud. What is a benefit to the snail of this change in response?
Question 25Paper ref 7(a)(i)AnalysisWhich statement correctly compares the wall of an artery with the wall of a vein and gives the reason?
Question 26Paper ref 7(a)(ii)AnalysisDuring exercise, why does the heart of a person with coronary heart disease respond less well than a healthy heart?
Question 27Paper ref 7(b)(ii)AnalysisThe chart above shows the relative risk of coronary heart disease (CHD) for non-smokers and smokers with few, medium or many risk factor alleles. Which conclusion is supported by the chart?
Relative risk of CHD
01234fewmediumhighRisk allelesRelative risk12333.54Non-smokersSmokers
Show the chart data as a table
Risk allelesNon-smokersSmokers
few13
medium23.5
high34
Question 28Paper ref 7(b)(ii)KnowledgeSmoking increases the risk of coronary heart disease in all groups. Which statement explains one way smoking does this?
Question 29Paper ref 8(a)(i)ApplicationLactose intolerance is caused by a recessive allele, d. The ability to digest lactose is caused by the dominant allele, D. A woman and a man both digest lactose normally, and each has a parent with lactose intolerance (dd). What is the probability that their child will be lactose intolerant?
Question 30Paper ref 8(a)(ii)AnalysisWhy do people who cannot digest lactose often get watery faeces (diarrhoea) after drinking milk?
Question 31Paper ref 8(a)(iii)AnalysisFewer adults are lactose intolerant in regions where milk is a major food all through life. Which statement explains this by natural selection?
Question 32Paper ref 8(b)(i)-(ii)ApplicationAn industrial yoghurt fermenter has a stirrer inside a tank, with a water jacket around the outside. Which row correctly gives the roles of the stirrer and the water jacket?
Question 33Paper ref 9(a)ApplicationA gene for efficient photosynthesis is to be placed into tomato plant cells using a plasmid vector. How is the recombinant plasmid made?
Question 34Paper ref 9(b)AnalysisTomato plants are grown at high light intensity. As temperature rises, the rate of photosynthesis first increases and then levels off at a steady value without falling. What is the most likely reason it levels off?
Question 35Paper ref 9(c)EvaluationIn a UK glasshouse, tomatoes are lit and heated using electricity from a wood-burning power station, and the station’s carbon dioxide is piped into the glasshouse. Which statement gives a correct advantage for farmers and for the environment?
Question 36Paper ref 10ApplicationA farmer wants to test whether the colour of a scarecrow’s clothes changes how well it keeps birds off a crop. Which plan is the best design?

Key points to remember

  • Red blood cells have cytoplasm but no nucleus and no cell wall.
  • Osmosis is the net movement of water from higher to lower water potential through a partially permeable membrane.
  • Magnification = image size ÷ actual size, after converting both to the same unit.
  • Alveoli are adapted by thin walls, a good blood supply that keeps a steep gradient, and moist surfaces.
  • Enzymes speed up with temperature until they reach the optimum, then denature when the active site changes shape.
  • Biomass in a food web rises and falls with light, nutrients and grazing.
  • Arteries have thick elastic and muscle walls for high pressure; veins have valves to stop backflow.
  • Natural selection needs variation (mutation), survival advantage, reproduction and passing on alleles.
  • A fair test changes one variable, controls the rest, measures an outcome and repeats.

Key terms

  • Osmosis: The net movement of water from a region of higher water potential to lower water potential through a partially permeable membrane.
  • Plasmolysis: The shrinking of the cytoplasm away from the cell wall when a plant cell loses water.
  • Magnification: How many times bigger an image is than the real object: image size ÷ actual size.
  • Denaturation: Permanent change in shape of an enzyme’s active site, usually caused by high temperature, so the substrate no longer fits.
  • Biomass: The mass of living material in an organism, population or trophic level.
  • Reflex arc: The pathway receptor, sensory neurone, relay neurone, motor neurone, effector that gives a fast automatic response.
  • Coronary heart disease: A condition in which fatty deposits narrow the coronary arteries and reduce blood supply to the heart muscle.
  • Recessive allele: An allele whose effect shows only when two copies are present, as in genotype dd.
  • Recombinant plasmid: A plasmid that has been cut open and had a foreign gene joined into it.
  • Limiting factor: The factor in shortest supply that stops the rate of photosynthesis rising further.

Frequently asked questions

How long is the Edexcel IGCSE Biology 4BI1 1BR paper?

The paper lasts 2 hours and is worth 110 marks in total. The marks shown in brackets beside each question are a guide to how much time to spend on it.

What equipment do I need for this paper?

The front page says you must have a ruler and a calculator. Several questions need calculations such as magnification, percentage increase, standard form and a mean, and one asks you to plot a graph.

Do I have to answer every question?

Yes. The instructions say to answer all questions in the spaces provided. There are 10 questions, and you should try every one and check your answers if you have time at the end.

What types of question appear on the paper?

The paper mixes single-mark multiple-choice questions, short calculations, graph plotting, and longer ‘explain’, ‘discuss’ and ‘design an investigation’ questions worth up to 6 marks.

Which topics does the May 2024 paper cover?

It covers cells and osmosis, flowers and cuttings, breathing and gas exchange, enzymes, food webs, reflexes, heart disease, genetics and natural selection, fermenters, genetic engineering, photosynthesis and experimental design.

What should you do next?

Re-read the explanation for every question you missed, then try the written paper under timed conditions and mark it with the official mark scheme from Pearson Edexcel. This quiz is independent practice material. It is not affiliated with or endorsed by Pearson Edexcel.

More practice: see all Biology Edexcel Past Paper Practice quizzes on this site.

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